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Consequently, the safety valve would be sized to pass at least 953 kg/h when set at 4 bar g. Once the fault load has been determined, it is usually sufficient to size the safety valve using the manufacturer’s capacity charts. A typical example of a capacity chart is shown in Figure 9.4.3.
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(The cooling rate in this apparatus is approximately -1°C/min.) The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial.
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